Year: 2018 | Month: June | Volume 8 | Issue 3
Timed Artificial Insemination Methods involving Ovulation of Second Follicular Wave vis-Ã -vis Extension in Growth of Dominant Follicle- New Alternatives to Enhance Fertility in Buffalo (Bubalus bubalis)
Abstract:
The study was undertaken to assess the ovarian and fertility responses following ovulation of dominant follicle (DF) from second follicular wave (2nd FW) through application of modified synchronization protocols in 46 cyclic postpartum buffaloes. All buffaloes were administered PGF2α (PGF; Cloprostenol, 500 mcg) on day -2, gonadotrophin releasing hormone (GnRH; Buserelin) on days 0 (10 mcg) and 7 (20 mcg). After that, all buffaloes were randomly divided into 2 groups on the basis of administration of second dose of PGF (500 mcg) on day 14 (Group I, n= 20) and 15 (Group II, n= 26). Third dose of GnRH (10 mcg) was administered on day 16 (Evening) and 17 (Morning) in group I and II, respectively, followed by artificial insemination (AI) at 12-16 h later. The pregnancy diagnosis was performed at day 45 post AI. Progesterone (P4) concentrations remained higher during 2FW in buffaloes that became pregnant compared to nonpregnant ones. A larger (P<0.01) diameter of the DF was observed on day of third GnRH and AI in group II compared to group I buffaloes. A comparable corpus luteum (CL) diameter was observed on all days of protocols in both groups. Ovulatory response following last GnRH administration and first AI pregnancy rate were 90 vs. 96 % and 35 vs. 46 % in group I vs. II, respectively. In conclusion, ovarian and fertility responses following ovulation of DF from 2nd FW showed an improved trend in buffaloes where growth of DF was extended by delaying the luteolysis.
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